#Scaffold protein download
#Scaffold protein free

Such reference data will be useful control data for all hydrogen exchange mass spectrometry experiments involving nanodiscs in which transmembrane or lipid-associated proteins are the primary molecule(s) of interest. Two major scaffold proteins (Sc proteins) have been identified, ScI and ScII. This work provides a catalog of the expected scaffold protein peptic peptides in a nanodisc-hydrogen exchange mass spectrometry experiment and their deuterium uptake signatures, data that can be used as a benchmark to verify correct assembly and nanodisc structure. Neuronal Scaffold Proteins Antibody Sampler Kit: Image 1 Western blot analysis of extracts from the indicated rat brain synaptic fractions using SHANK2. The rapid deuteration of the scaffold protein and the presence of correlated unfolding events both indicate that nanodiscs are dynamic rather than rigid bodies in solution. Several regions of the scaffold protein, in both the lipid-free and lipid-associated states, displayed EX1 unfolding kinetics. This protection was short-lived and the scaffold protein was highly deuterated within 2 h. On nanodisc self-assembly, the entire scaffold protein gained significant protection from exchange, consistent with a large, protein-wide, structural rearrangement. Hydrogen exchange mass spectrometry was used to probe the structure and dynamics of the scaffold protein in the presence and absence of lipid. Nanodiscs consist of a discoidal phospholipid bilayer encircled by two copies of an amphipathic alpha helical membrane scaffold protein, which is modeled from apolipoprotein A-1. Phospholipid bilayer nanodiscs are model membrane systems that provide an environment where membrane proteins are highly stable and monodisperse without the use of detergents or liposomes.Xiaomei Han at the Qian's Bioinformatics Lab, Johns Hopkins School of Medicine.

#Scaffold protein download

Users for commercial purpose please contact the authors before download the data sets.

#Scaffold protein free

The content in the database is free to academic and non-profit organizations. Heng Zhu, Jin Zhang, and Jiang Qian's labs. Although not strictly defined in function. Scaffold proteins are crucial regulators of many key signaling pathways. The datasets were generated from a collaborative project of Drs. Definition: Binding to a scaffold protein. The Experimental Scaffold Proteins and Signaling pathways The Predicted Scaffold Proteins and Signaling Pathways phosphorylating a related scaffold protein, SLP-76 and LAT.16 These scaffold proteins. Systematic Prediction of Scaffold Proteins Reveals New Design Principles in Scaffold-Mediated Signal Transduction. phosphorylating SLP-65, a scaffolding protein.15 Similarly. Hu J, Neiswinger J, ZHANG J, Zhu H, Qian J (2015). These proteins usually have many protein binding domains (like WD40 repeats). Protein View Input a scaffold protein or pathway protein(e.g. Scaffold protein A protein whose main function is to bring other proteins together for them to interact. ScaPD contains experimental scaffold proteins, predicted scaffold proteins and signaling pathways.Ĭlick here for frequently asked questions.